Genetic characterization of fermentation yeasts as an element of geographical indication of ‘cachaça’ and ‘aguardente’ from Luiz Alves, SC

Authors

DOI:

https://doi.org/10.52945/rac.v34i3.1104

Keywords:

Geographic identification, molecular markers, genetic differentiation

Abstract

The municipality of Luiz Alves/SC maintains familiar traditions of ‘cachaça’ and ‘aguardente’ production by artisanal alembics, demanding Geographic Identification processes for the valorization of local products and protection of associated traditional knowledge. Aiming to subsidize the Geographical Identification of the products, the objective of this work was to characterize, by molecular basis, the native yeasts used by six alembics in the region. Analysis of three genomic regions (ITS, LSU and interdelta) were performed for comparison with the commercial yeast UFLA CA-11. Despite the similarity (BLAST) of the LSU/ITS sequences of all yeasts with Saccharomyces cerevisiae, both phylogenetic inferences and principal component analyzes recovered important genetic differentiation in relation to commercial yeast, along with the uniqueness between samples from different establishments. The results present herein are pioneering in molecular characterization of yeasts used in production processes of cachaça in SC aiming Geographic Identification of Luiz Alves products and highlights the potential use of those molecular markers in the process.

Metrics

Metrics Loading ...

Author Biographies

Valdir Marcos Stefenon, UFSC/CCA

Biólogo, PhD, UFSC/CCA, Rod. Admar Gonzaga, 1346, 88034-000, Florianópolis, SC, fone (48) 3721-5331

Bruna Ronchi Hermann, UFSC/CCA

Acadêmica do Curso de Agronomia

Julia Zappelini, UFSC/CCA

Engenheira Agrônoma, M.Sc.

Milena Martins Machado, UFSCS/CCA

Acadêmica do Curso de Agronomia

References

ARAÚJO, T.M., SOUZA, M.T.; DINIZ, R.H.S.; YAMAKAWA, C.K.; SOARES, L.B.; LENCZAK, J.L.; DE CASTRO OLIVEIRA, J.V.; GOLDMAN, G.H.; BARBOSA, E.A.; CAMPOS, A.C.S.; CASTRO, I.M.; BRANDÃO, R.L. Cachaça yeast strains: alternative starters to produce beer and bioethanol. Antonie van Leeuwenhoek, v.111 n.10, p.1749-66, 2018. DOI: https://doi.org/10.1007/s10482-018-1063-3

BARBOSA, E.A.; SOUZA, M.T.; DINIZ, R.H.S.; GODOY-SANTOS, F.; FARIA-OLIVEIRA, F.; CORREA, L.F.M.; ALVAREZ, F.; COUTRIM, M.X.; AFONSO, R.J.D.C.F.; CASTRO, I.D.M.; BRANDÃO, R.L. Quality improvement and geographical indication of cachaça (Brazilian spirit) by using locally selected yeast strains. Journal of Applied Microbiology, v.121, n.4, p.1038–1051, 2016. DOI: https://doi.org/10.1111/jam.13216

BADOTTI, F.; BELLOCH, C.; ROSA, C.A.; BARRIO, E.; QUEROL, A. Physiological and molecular characterisation of Saccharomyces cerevisiae cachaça strains isolated from different geographic regions in Brazil. World Journal of Microbiology and Biotechnology, v.26, n.4, p.579-587, 2010.

BRASIL. Presidência da República. Casa Civil. Decreto nº 6.871, de 4 de junho de 2009. Brasília, DF, 2009.

FERREIRA-D’SILVA, A.; BADOTTI, F.; PINHEIRO, C.S.R.; GONÇALVES, C.M.; HUGHES, F.M.; GÓES-NETO, A.; UETANABARO, A.P.T. Diversity of Saccharomyces cerevisiae strains isolated of the spontaneous fermentation of cachaça from northeastern Brazil. Brazilian Journal of Development, v.5, n.11, p.27448-2746, 2019.

HAMMER, Ø.; HARPER, D.; RYAN, P.D. Past: paleontological statistics software package for education and data analysis. Palaeontologia Electronica, v.4, p.1-4, 2001.

HOANG, M.T.V.; IRINYI, L.; CHEN, S.C.A.; SORRELL, T.C.; THE ISHAM BARCODING OF MEDICAL FUNGI WORKING GROUP; MEYER, W. Dual DNA Barcoding for the Molecular Identification of the Agents of Invasive Fungal Infections. Frontiers in Microbiology, v.10, n.1647, 2019. DOI: https://doi.org/10.3389/fmicb.2019.01647

Published

2021-12-28

How to Cite

Marcos Stefenon, V., Ronchi Hermann, B., Zappelini, J., & Martins Machado, M. (2021). Genetic characterization of fermentation yeasts as an element of geographical indication of ‘cachaça’ and ‘aguardente’ from Luiz Alves, SC. Agropecuária Catarinense Journal, 34(3), 27–29. https://doi.org/10.52945/rac.v34i3.1104

Issue

Section

Scientific note

Most read articles by the same author(s)

Publication Facts

Metric
This article
Other articles
Peer reviewers 
2
2.4

Reviewer profiles  N/A

Author statements

Author statements
This article
Other articles
Data availability 
N/A
16%
External funding 
N/A
32%
Competing interests 
N/A
11%
Metric
This journal
Other journals
Articles accepted 
33%
33%
Days to publication 
347
145

Indexed in

Editor & editorial board
profiles
Publisher 
Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri