Aseptic techniques for cleaning bulbs of some ornamental plants grown in vitro
Keywords:
in vitro culture , contamination, ornamental plantsAbstract
Cultivation of tissue explants from bulbs, rhizomes and stolons often finds high rates of contamination by bacteria, fungi or other organisms. In order to establish an aseptic procedure for introducing into in vitro culture of hyacinth, tulip, iris and narcissus, with special emphasis on hyacinth, a series of tests were carried out.
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References
BERGOÑON, S.; RIEIRA, R.; SELLES, M; ROIG, N.; CODINA, C. Efecto de diferentes tratamientos en la asepsia de explantos en especies de
Narcissus y su influencia en la regeneración de brotes. In: REUNIÓN NACIONAL DE LA S.E.F.V., 10 y CONGRESO HISPANO-PORTUGUES DE FISIOLOGIA VEGETAL, 3., 1993, Pamplona. Anales. Pamplona: 1993.
HOLG, G.M.C.; VAN der LINDE, P.C.G. Reduction of contamination in bulbexplant cultures of Narcissus by a hot-water treatment of parent bulbs. Plant Cell, Rockville, v.31, p.75-79, 1992.
KUNNEMAN, B.P.A.M.; ALBERS, M.R.J. Snelheid grootste struikelblok; weefselkweek pioen gaat niet over rozen. Bloembollencultuur, v.100, n.23, p.16-17, 1989.
MARGARA, J. Multiplicación vegetativa y cultivo in vitro; los meristemos y la organogénesis. Madrid: Mundi-Prensa, 1988. 232p.
MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, Copenhagen, v.15, p.473-479, 1962.
PAEK, K.Y.; THORPE, T.A. Hyacinth. In: AMMIRATO, P.V.; EVANS, D.R.; SHARP, W.R.; BAJAJ, Y.P.S. Handbook of plant cell culture. New York: McGraw-Hill, 1990. v.5, p.439-471.
TYNAN, J.L.; CONNER, A.J.; MACKNIGHT, R.C.; POULTER, R.T.M. Miconazole: an effective antifungal agent for plant tissue culture. Plant Cell, Rockville, v.32, p.293-301, 1993.
VAN der LINDE, P.C.G.; SCHIPPER, J.A. Micropropagation of Iris with special reference to Iris x hollandica Tub. In: BAJAJ, Y.P.S. Biotechnology in agriculture and forestry. Berlin: Springer-Verlag, 1992. v.20, p.173-197.
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Copyright (c) 1997 Mario Angelo Vidor
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