In vitro vine propagation: production of basic plants for rootstocks

DOI: 10.52945/rac.v16i3.1029
Published 2003-07-28

Authors

  • Aparecido Lima da Silva
    CCA/ UFSC
  • Enio Schuck
    Epagri/Estação Experimental de Videira
  • Robison Borges
    cidasc
  • Flávia Maia Moreira
    Istituto San Michele all’Adige
  • Marcelo Borghezan
    UFSC/CCA
  • Liziane Kadine Antunes de Moraes
    UFSC/CCA- Recursos Genéticos Vegetais
  • Célio Air Mikulski
    Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina
  • Carolina Quiumento Velloso
    UFSC/CCA

Abstract

Techniques in vitro are useful in order to generate basic material with high genetic quality and
adequate sanitary standards. The present study aimed at the in vitro production of vine rootstocks to evaluate morphological parameters during micropropagation. Axillar buds of ‘Paulsen 1103’, ‘VR043-43’ and ‘Gravesac’ rootstocks were inoculated on DSD1 culture medium. The rate of responsive cultures was 65% and in in vitro
condition the leaf area and the dry weight were different among the genotypes, being the rootstock ‘Paulsen 1103’ the best one in terms of number of leaves, length of roots, leaf area and biomass yield. ‘Gravesac’ presented the best results for stem length and root number. The rate of survival in the acclimatization was 95%. The evaluated
rootstocks showed adequate morphologic features for micropropagation and acclimatization.

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Keywords

  • Vitis
  • micropropagation
  • certified plant stock