Susceptibility of Pacific white shrimp to Vibrio alginolyticus infection under different oxygen saturations

DOI: 10.52945/rac.v28i2.163
Published 2015-01-27

Authors

  • Mauricio Lehmann
    Pesquisador do Núcleo de Pesquisa em Pesca e Aquicultura (Nupa Sul-1) do Instituto Federal Catarinense – campus Araquari (IFCCA), BR-280, Km 27, 89245-000 Araquari, SC
  • Delano Dias Schleder
    Pesquisador do Nupa Sul-1 do IFCCA
  • Adolfo Jatobá
    Pesquisador do Nupa Sul-1 do IFCCA
  • Luciane Maria Perazzolo
    Pesquisadora do Laboratório de Imunologia Aplicada à Aquicultura (LIAA) da Universidade Federal de Santa Catarina (UFSC), 88040-900 Florianópolis, SC
  • Luis Vinatea
    Pesquisador do Laboratório de Camarões Marinhos (LCM) da UFSC, Beco dos Coroas, 503, 88061-600 Florianópolis, SC

Abstract

Abstract: The present study aimed to evaluate the cumulative mortality, reactive oxygen species production (ROS) and total hemocyte counts (THC) of the marine shrimp Litopenaeus vannamei infected with Vibrio alginolyticus at different oxygen saturations. A total of 240 shrimp (10 ± 1.5g) were distributed into 16 tanks (60L), divided into two groups, infected and uninfected, which were subjected to 30 or 100% of saturation (in quadruplicate). During 72 hours after infection, daily hemolymph samples were collected for THC and ROS evaluation, and dead animals were removed and computed to assess cumulative mortality rates. Mortality was observed only in infected group, and was higher under 30% of saturation (97.2%) than 100% (77.8%), however, no significant differences were observed between THC and ROS production of infected and uninfected animals, under both saturations. Therefore, the results of this study suggested that hypoxia increases the susceptibility of the shrimps to vibrio infection, which may be not related to the modulation of THC and ROS production.

Author Biographies

  • Mauricio Lehmann
    Médico-veterinário, Dr.
    • Delano Dias Schleder
      Biólogo, M.Sc.
      • Adolfo Jatobá
        Engenheiro de aquicultura, Dr.
        • Luciane Maria Perazzolo
          Bióloga, Dra.
          • Luis Vinatea
            Biólogo, Dr.
              Competing Interests

                

               

               

               

               

               

               

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            Keywords

            • Litopenaeus vannamei
            • dissolved oxygen saturation
            • bacterial infection.