First report of Fusarium solani in soybeans (Glycine max) in Misiones, Argentina

DOI: 10.52945/rac.v39i.2373
Published 2026-11-10

Authors

Abstract

Sudden death syndrome (SDS) in soybeans is caused by various Fusarium species in Argentina, grouped under the F. solani complex. This disease can cause yield losses of up to 50%.  To demonstrate morphologically and genetically that Fusarium solani causes sudden death syndrome in soybeans in the province of Misiones, Argentina. Soybean root isolation was performed at the Phytopathology Laboratory of the National University of the Northwest of Buenos Aires Province, using sporodochia (sign) from the Cerro Azul Agricultural Experiment Station, Misiones, Argentina, during 2022. The isolation was obtained using potato dextrose agar (pH 5.5) culture medium under a laminar flow hood, incubated in a growth chamber (24°C ± 2, 12 hours of light followed by 12 hours of darkness). DNA was extracted from the mycelium for molecular analysis. A PCR product corresponding to the translation elongation factor (TEF) gene was amplified. For pathogenicity testing, Koch's postulates were performed. Soybean seedlings at the V1 stage were disinfected according to the method described by Hernández. The seedlings were inoculated with Fusarium. The Fusarium species was inoculated in triplicate, and a control unit was also included. These studies were conducted at the Phytopathology Laboratory of UNNOBA during 2022. Molecular results demonstrated that the species causing root rot in soybeans was F. solani. This is the first molecular report of this Fusarium species causing root rot in soybeans in the province of Misiones, Argentina.

Author Biographies

  • miguel

    Ingeniero agronomo. Master of Science in Vegetal Protection. Doctor en Ciencias Agrarias. Mestre em Produção Vegetal (UFAC). E-mail: miguellavillapergamino@gmail.com

    • Conceptualization
    • Methodology
    • Formal Analysis
    • Investigation
    • Writing – Review & Editing
    Competing Interests

    fitopatologia 

  • Ana Edith Fernández

    Licenciada en Genética graduada de la Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA). Gracias a mi vocación y formación en el área vegetal considero que me habilitan para participar en múltiples actividades asociadas al mejoramiento genético, caracterización genética de materiales y utilización de técnicas moleculares. 

    • Investigation
    • Writing – Review & Editing
    Competing Interests

    No conflict of interest.

References

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Keywords

  • sudden death syndrome
  • sporodochia
  • mycelium
  • translation elongation factor